Magnesium Lithospermate B, an Active Extract of Salvia miltiorrhiza, Mediates sGC/cGMP/PKG Translocation in Experimental Vasospasm
Figure 2
Inhibitory effect of MLB on RhoA translocation (a) and ROCK-II expression (b) in the BAs. Expression of RhoA in the cytoplasm and membrane as well as ROCK-II was also determined by Western blot analysis. (A) the healthy controls, (B) the vehicle-treated SAH rats, (C) SAH rats received 10 mg/kg/day MLB treatment, (D) SAH rats received 10 mg/kg/day MLB preventive treatment, and SAH rats received both 10 mg/kg/day MLB pretreatment and 1 mg/kg L-NAME as (E) (upper panel). The ratio of membrane bound to cytoplasm RhoA in the SAH group was set at 100% in (a), whereas the expression of ROCK-II (normalized using -actin) was set the same in (b). Data are mean ± SEM (/group). , versus the vehicle +SAH, MLB and L-NAME groups, respectively.
