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BioMed Research International
Volume 2014, Article ID 279408, 6 pages
Research Article

Engineered Tumor Cell Apoptosis Monitoring Method Based on Dynamic Laser Tweezers

1Key Laboratory of Optoelectronic Information Science & Technology, Institute of Modern Optics, Nankai University, Ministry of Education of China, Tianjin 300071, China
2Institute of Oncology, Tianjin Union Medicine Centre, Tianjin 300121, China
3Optics Research Group, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
4Institute of Micro & Nano Optics, Shenzhen University, Shenzhen 518060, China

Received 22 January 2014; Revised 10 March 2014; Accepted 10 March 2014; Published 1 April 2014

Academic Editor: Shiwu Zhang

Copyright © 2014 Yuquan Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Monitoring the cells’ apoptosis progression could provide a valuable insight into the temporal events that initiate cell death as well as the potential for rescue of apoptotic cells. In this paper, we engineered a novel and robust method for monitoring apoptosis of tumor cells based on dynamic laser tweezers, using A549 and HeLa cell line as typical samples. The entire experiment can be completed in a few hours with small amount of fluid sample, presenting great advantages of celerity, microscaled measurement, and label-free explorations without perturbing experimental conditions in combination with other probes. Validity and stability of this method are verified experimentally in terms of physical parameters of the system. The proposed technique has great potential in improving cancer treatment by monitoring the objective efficacy of tumor cell killing.