Research Article
Preliminary Evaluation of Probiotic Properties of Lactobacillus Strains Isolated from Sardinian Dairy Products
Table 3
Enzymatic profiles of selected dairy Lactobacillus strains assayed by the API-ZYM system.
| | Enzyme tested | Strains | | L. paracasei | L. plantarum | L. brevis | | 62LP39 | 1A6M | 11/20966 | 19/20711 | 4/116868 | 1C3M |
| | Alkaline phosphatase | 20 | 10 | 10 | 5 | 10 | 5 | | Esterase (C4) | 10 | 5 | 5 | 0 | 5 | 20 | | Esterase lipase (C8) | 10 | 10 | 5 | 10 | 10 | 10 | | Lipase (C14) | 10 | 20 | 10 | 10 | 20 | 20 | | Leucine arylamidase | >40 | >40 | >40 | 30 | >40 | >40 | | Valine arylamidase | >40 | >40 | 30 | 10 | 30 | >40 | | Cystine arylamidase | 20 | 20 | 10 | 0 | 20 | 20 | | Trypsin | 5 | 5 | 0 | 0 | 5 | 0 | | α-Chymotrypsin | 0 | 0 | 5 | 5 | 5 | 5 | | Acid phosphatase | 20 | 30 | 20 | 20 | 20 | 30 | | Napthol-AS-BI-phosphohydrolase | 30 | 20 | 10 | 10 | 10 | 10 | | α-Galactosidase | 20 | 10 | 10 | 0 | 0 | 30 | | β-Galactosidase | >40 | 30 | >40 | 10 | 20 | >40 | | β-Glucuronidase | 0 | 0 | 0 | 0 | 0 | 0 | | α-Glucosidase | 30 | 30 | 20 | 0 | 20 | 30 | | β-Glucosidase | 30 | 10 | 30 | 20 | 30 | 30 | | N-Acetyl-glucosaminidase | 5 | 0 | 0 | 5 | 0 | 0 | | α-Mannosidase | 0 | 0 | 0 | 0 | 0 | 0 | | α-Fucosidase | 0 | 5 | 0 | 0 | 0 | 0 |
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Enzyme activity is expressed as the approximate nanomoles of hydrolysed substrate after 4 h of incubation at 37°C.
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