Expression of Acetylcholine Receptors by Experimental Rat Renal Allografts

Table 1

Primer sequences used for real-time PCR.

Gene	Accession number	Direction	5′-3′ sequence	Product (bp)
CHRM1	NM_080773	Forward	tgtggccagcaacgcctctg	106
		Reverse	ccttcggggagtgcgcttgg
CHRM2	NM_031016	Forward	gcccaacccaccacgagcc	102
		Reverse	agttgtggccgctgcggaa
CHRM3	NM_012527	Forward	gtccctcggaggcagggct	116
		Reverse	acccccgagagggtcgctg
CHRM4	NM_031547	Forward	cgactcgcggaacctctggc	117
		Reverse	ggcgtgaagttggccatgctg
CHRM5	NM_017362	Forward	ccacagcaaagtcgatgaggca	102
		Reverse	tttggcctcccctcttcttggc
CHRNA2	NM_133420	Forward	cacggccagtgcccaacact	119
		Reverse	ctgctttagccagacattggtggt
CHRNA3	NM_052805	Forward	tgggtgttgtgctgctcccg	124
		Reverse	actggaacaggcggtgctca
CHRNA4	NM_024354	Forward	agggaccggcctcttgcctg	113
		Reverse	tgcctactggccgagaccac
CHRNA5	NM_017078	Forward	accagctgatgacgacgaacg	117
		Reverse	acagggagtccgaaggaacacg
CHRNA6	NM_057184	Forward	ctttgagttggccatcacgc	116
		Reverse	ccgttggatcccaacacaatc
CHRNA7	NM_000746.4	Forward	agatggccagatttggaaacc	142
		Reverse	gcaggaactcttgaatatgcct
CHRNA9	NM_022930	Forward	atctggtgtggaggccggaca	119
		Reverse	ggccggtgagtcccaggtgat
CHRNA10	NM_022639	Forward	accagtggcagatacagaccagac	124
		Reverse	tgtccactcttgccggatccac
CHRNB2	NM_019297	Forward	gtccggctcccttccaaacaca	114
		Reverse	gctgccatcataggagaccacagc
CHRNB4	NM_052806	Forward	ccgcctggagctatcactgtcc	110
		Reverse	tccaggccaggcggtagtca
PBGD	NM_013168	Forward	ggcgcagctacagagaaagt	115
		Reverse	agccaggataatggcactga