BioMed Research International / 2014 / Article / Fig 4

Research Article

Sensitization of Cancer Cells through Reduction of Total Akt and Downregulation of Salinomycin-Induced pAkt, pGSk3β, pTSC2, and p4EBP1 by Cotreatment with MK-2206

Figure 4

Cotreatment with MK-2206 reduced the levels of p70S6K, mTOR, and PDK1 activated forms and reduced Sal-activated GSk3 , TSC2, and 4EBP1. (a–c) Hs578T cell extracts were collected at 24 h after treatment with 0.5 μM MK-2206, 5 μM Sal (Sal), 0.5 μM MK-2206 with 5 μM Sal (MK + Sal), or DMSO (Con). The cells were used for Western blot analyses using antibodies against phosphorylated mTOR, mTOR, phosphorylated p70S6K, phosphorylated PDK1, phosphorylated GSK3 , phosphorylated TSC2, phosphorylated 4EBP1, phosphorylated PTEN, PTEN, PI3K, FOXO1, Survivin, Cyclin D1, CDK4, PCNA, pRb, and GAPDH. (d) Hs578T cell extracts were collected at 24 h after treatment with 1 μM MK-2206, 5 μM Sal (Sal), 1 μM MK-2206 with 5 μM Sal (MK-1 + Sal), or DMSO (Con). The cells were used for Western blot analyses using antibodies against phosphorylated mTOR, phosphorylated p70S6K, phosphorylated GSK3 , phosphorylated TSC2, phosphorylated 4EBP1, Cyclin D1, pRb, and GAPDH.
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