BioMed Research International / 2014 / Article / Fig 5

Research Article

Sensitization of Cancer Cells through Reduction of Total Akt and Downregulation of Salinomycin-Induced pAkt, pGSk3β, pTSC2, and p4EBP1 by Cotreatment with MK-2206

Figure 5

Sal and MK-2206 cotreatment sensitization mechanism is also conserved in KB cancer cell line. (a) KB cell extracts were collected at 24 h after treatment with 0.5 μM MK-2206, 5 μM Sal (Sal), 0.5 μM MK-2206 with 5 μM Sal (MK + Sal), or DMSO (Con). (b) Cotreatment with LY294002 reduced Sal-activated GSk3 , TSC2, and 4EBP1. Hs578T cell extracts were collected at 24 h after treatment with 5 μM Sal (Sal), 20 μM LY294002, 5 μM Sal with 20 μM LY294002 (Sal + LY), or DMSO (Con). The cells were used for Western blot analyses using antibodies against C-PARP, phosphorylated TSC2, phosphorylated GSK3 , phosphorylated Akt, Akt, phosphorylated 4EBP1, Survivin, pRb, and GAPDH.

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