Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration
Immunohistochemistry staining images of CD31 for implants in vivo. Results show that human CD31 positive-expressing lumens (white arrows) and some invading host blood vessels (green arrows) were observed in HUVEC/hMSC constructs at 1 week (a) and 2 weeks (c), but there were only invading host blood vessels (green arrows) in the nonprevascularized group ((b), (d)). A magnified image from (c) shows that the human derived lumen (brown color) carried murine blood cells (e). Quantitative result shows the density of the human CD31 positive-expressing in the HUVEC/hMSC constructs at 1 and 2 weeks, but there is no significant difference between 1 and 2 weeks (f). (Scale bar = 100 μm).