Carbapenemase Genes among Multidrug Resistant Gram Negative Clinical Isolates from a Tertiary Hospital in Mwanza, Tanzania

Table 1

Primer sets for amplification of carbapenem resistance determine genes (16).

Gene	Primer sequence (5′→ 3′)	TM (°C)	Amplicons size (bp)
bla-VIM	Forward: GATGGTGTTTGGTCGCATA; Reverse: CGAATGCGCAGCACCAG	54.5; 57.6	390
bla-KPC	Forward: CATTCAAGGGCTTTCTTGCTGC; Reverse: ACGACGGCATAGTCATTTGC	60.3; 57.3	498
bla-NDM	Forward: GGTTTGGCGATCTGGTTTTC; Reverse: CGGAATGGCTCATCACGATC	57.3; 59.4	521
bla-IMP-1	Forward: TTGACACTCCATTTACAG; Reverse: GATTGAGAATTAAGCCACTCT	49.1; 54.0	232
bla-IMP-2	Forward: TTGACACTCCATTTACGG; Reverse: GATCGAGAATTAAGCCACCCT	51.4; 57.9	232
bla-IMP-3	TTGACACTCCATTTACTG; GATCGAGAATTAAGCCACTCT	49.1; 55.9	232
bla-OXA-48	Forward: GCTTGATCGCCCTCGATT; Reverse: GATTTGCTCCGTGGCCGAAA	56.0; 59.4	238

TM: melting temperature of the primer.