BioMed Research International / 2014 / Article / Tab 1

Research Article

Immobilization of a Pleurotus ostreatus Laccase Mixture on Perlite and Its Application to Dye Decolourisation

Table 1

Effects of experimental parameters on laccase immobilization yield on perlite. NaC: 50 mM sodium citrate buffer; McI: McIlvaine’s buffer. *Incubations were performed in 50 mM sodium phosphate buffer, unless otherwise is indicated. Reported data are the mean values of three independent experiments. The standard deviation of tests carried out at a given set of operating conditions was less than ±10%.

RunInitial activity (IU/g)Initial protein (mg/g)Glutaraldehyde
% vol
pH*Incubation time and temperatureImmobilized activity (IU/g)Immobilization yield (%)

R15504.516.54 h RT20036
R25504.516.5Overnight 4°C12823
R35504.52.56.54 h RT20637
R45504.52.56.5Overnight 4°C15127
R55504.556.54 h RT20637
R65504.556.5Overnight 4°C20738
R75507.7516.51 h RT16029
R85507.7516.54 h RT16129
R95507.750.56.51 h RT18634
R105507.750.56.54 h RT17832

R11801.30.56.51 h RT5670
R122854.750.56.51 h RT13648
R1380011.250.56.51 h RT19925

R145004.250.56.51 h RT17335
R15500120.56.51 h RT17335
R1650022.50.56.51 h RT18437

R172752.250.5NaC 5.51 h RT5219
R182752.250.56.51 h RT13750
R192752.250.57.51 h RT12043
R202752.250.5McI 5.51 h RT3212
R212752.250.5McI 7.51 h RT6624
R222752.250.5McI 6.51 h RT4215

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