Figure 1: Reactivity of the MAbs with denatured recombinant CA XII expressed in E. coli and mammalian cells. Upper-leftmost panel: SDS-PAGE; remaining panels: immunoblot with MAbs 1D5, 4A6, 5D2, 8C9, 9A8, 13F5, and 15A4. Line M: prestained MW markers (Thermo Fisher Scientific, Vilnius); line 1: purified recombinant CA XII proteinexpressed in E. coli; line 2: lysate of E. coli Rosetta (DE3) strain cells; line 3: purified recombinant CA XII protein expressed in mammalian cells.