Development and Characterization of New Monoclonal Antibodies against Human Recombinant CA XII

Table 1

PCR primers used to produce three overlapping segments of CA XII.

Segment number	Segment sequence	PCR primer sequences*	Restriction endonuclease
#1	Val 27-Gly130	5′ CCCGGGATCCGTGAACGGTTCCAAG 3′	BamHI
		5′ ACGGCGGCCGCTTAGCCGTGCGGGTCATT 3′	NotI
#2	Ser111-Leu210	5′ GGGCGGATCCTCTCGCTACAGTGCC 3′	BamHI
		5′ GTCCTCTCGAGTTACAGCTCTTCAATGTTG 3′	XhoI
#3	Asp184-Ser290	5′ TCCGGGATCCGACAAGATCTTCAGTC 3′	BamHI
		5′ AGACCTCGAGTTAGGAGAAGGAGGTGTATAC 3′	XhoI

∗Restriction endonuclease recognition sites are underlined.