Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
Figure 1
Phenotype caused by pax1b knockdown. In order to analyze the function of pax1b during embryogenesis, we injected pax1b-specific morpholinos into fertilized eggs to block production of functional Pax1b protein. (a) Embryo injected with 5 ng control morpholino. ((b)-(c)) The phenotypes of pax1b morphants caused by 2 ng or 5 ng injection doses. ((d)–(g)) The phenotypes caused by coinjection of 200 ng pax1b or gfp mRNA with 5 ng pax1b MO or control MO. pax1b mRNA could partially rescue the defective fin bud phenotype of the pax1b morphant (g); gfp mRNA failed to rescue defective fin bud phenotype of the pax1b morphant (e). (h) Statistical analysis of phenotypes caused by coinjection of different mRNAs with 5 ng pax1b MO. 150 embryos were calculated. The amount of mRNA injected for every embryo is as follows: 200 ng gfp mRNA, 200 ng pax1b mRNA, 200 ng pax1a mRNA, 150 ng amphioxus pax1/9 mRNA, and 200 ng zebrafish pax9 mRNA. All embryos were observed at 24 hpf. zpax1b: zebrafish pax1b, zpax1a: zebrafish pax1a, zpax9: zebrafish pax9. hpf: hours post-fertilization. gfp: green fluorescence protein. ctr: control.
