Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development

Figure 3

Fin bud defects caused by pax1b knockdown. ((a)–(d)) Dorsal view of the region near the fin bud with orientation of head towards the top. (a) Control embryos. ((b)-(c)) pax1b morphants. (d) Rescue embryos: coinjection with 5 ng pax1b MO and 200 ng pax1b mRNA. (e) Statistical analysis of fin bud defects caused by injection of control MO or pax1b MO. Total numbers of injected embryos are labeled. ((f)–(m)) Expression pattern of erm and pea3 in control embryos, pax1b morphants, and coinjected embryos. Numbers of defective embryos and total numbers of stained embryos are labeled in the bottoms. The embryonic stage is 72 hpf in panels (a)–(e) and 32 hpf in panels (f)–(m). All the embryos are viewed from the dorsal side with head towards the top.

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