Characterization of Glial Cell Models and <i>In Vitro</i> Manipulation of the Neuregulin1/ErbB System

<table>Following lentivirus (LV) infection, RT4-D6P2T successfully express GFP and ecto-ErbB4-FLAG<i>.</i> Confluent cells infected with<i> LV-sGFP</i> only (Panels (a)–(d)) or with<i> LV-sGFP</i> and<i> LV-ecto-ErbB4-FLAG</i> (Panels (e)–(h)) were stained with anti-GFP (green) and anti-FLAG (red) antibody. Nuclei were stained with Hoechst (blue). Scale bar 100 <svg height="9.60567pt" id="M90" style="vertical-align:-3.53608pt" version="1.1" viewbox="0 -6.06959 7.51925 9.60567" width="7.51925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M538 96L524 119C492 88 454 63 446 63C439 63 434 70 440 101C463 223 491 341 518 448H508L433 422L401 276C355 192 240 56 188 56C163 56 154 89 163 133C184 233 207 338 235 443L230 448L152 424L58 17C40 -60 23 -143 23 -185C23 -241 42 -261 63 -261C92 -261 117 -241 125 -227L124 -221C109 -209 89 -165 89 -103C89 -55 96 -13 105 26H107C121 -3 134 -12 151 -12C172 -12 194 -5 221 16C279 61 330 124 384 194H386C381 159 377 141 368 105C343 3 363 -12 383 -12C416 -12 486 35 538 96Z" id="g113-234"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="555" vert-adv-y="555"></glyph.data></g></svg>m. Panel (i)-Western blot analysis of RT4-D6P2T infected with LV-sGFP only, or with LV-sGFP and LV-ecto-ErbB4-FLAG. (j) Recombinant ecto-ErbB4-FLAG peptide was purified from RT4-D6P2T conditioned medium using ANTI-FLAG affinity gel and eluted using FLAG peptide. Eluted fractions were analyzed by Western blot to identify the positive fractions to be frozen and used for the following experiments.</table>

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Figure 8

Figure 8: Characterization of Glial Cell Models and <i>In Vitro</i> Manipulation of the Neuregulin1/ErbB System 

BioMed Research International

Characterization of Glial Cell Models and In Vitro Manipulation of the Neuregulin1/ErbB System

Figure 8