Research Article

Characterization of Glial Cell Models and In Vitro Manipulation of the Neuregulin1/ErbB System

Figure 9

Ecto-ErbB4 stimulates ERK phosphorylation in NOBEC. Western blot analysis of NOBEC cells stimulated with recombinant soluble ecto-ErbB4-FLAG for 0, 5, 10, 15, 30, and 60 min. Western blot was analyzed with antibodies anti-p-AKT and AKT (Panel (a)) and anti-p-ERK 1/2 and ERK (Panel (b)). (c) Bands were analyzed by quantifying the intensity of the pixels per (Image J). The values of the bands corresponding to phosphorylated proteins were normalized to the intensity of the bands corresponding to the total proteins.
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(a)
310215.fig.009b
(b)
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(c)