Andrographolide Induces Apoptosis of C6 Glioma Cells via the ERK-p53-Caspase 7-PARP Pathway

Figure 3

p53 and its downstream molecules were involved in AND-induced apoptosis in C6 glioma cells. (a) The expression of p-p53 and p53. Cells were treated with 15 μM AND for 0, 6, 12, or 24 h, and cell lysates were analyzed for total p53 and p-p53. 𝑁=3. (b) DAPI stain. Cells were treated with 0.1% DMSO, 15 μM AND, or 15 μM AND plus 15 μM pifithrin-α for 12 h and stained with DAPI. Bar = 20 μM. Data is quantitated by cell counting. 𝑁=4. (c) The protein expression of c-PARP and p-caspase 7. Cells were treated with 0.01% DMSO (CTL), 15 μM AND with or without 15 μM pifithrin, or pifithrin alone for 24 h, and cell lysates were analyzed for cleaved PARP (c-PARP) and c-caspase 7. ^∗𝑃<0.05, ^∗∗𝑃<0.01, as compared to the 0 h or CTL group, respectively. ^#𝑃<0.05, ^##𝑃<0.01, as compared to the AND-group. 𝑁=4.