Figure 9: Representative optical microscopic images of hABMSCs after Alizarin red staining treatment with static condition (a1, b1) or at 10 min/day (a2, b2), 30 min/day (a3, b3), 60 min/day (a4, b4), 120 min/day (a5, b5), and 180 min/day (a6, b6) by OSS treatment with OM on 5 days and 10 days, respectively. OSS groups of 10, 30, and 60 min/day were intense compared to those of control. Representative microscopic images of hABMSCs after Von-Kossa staining with static condition (c1, d1) or at 10 min/day (c2, d2), 30 min/day (c3, d3), 60 min/day (c4, d4), 120 min/day (c5, d5), and 180 min/day (c6, d6) by OSS treatment with OM. Mineralized nodule as optical density measured after destaining treatment (C). OSS induction exposed at 10, 30, and 120 min/day groups was significantly different ( ) among groups ( , bar = 1 mm).