Research Article
Synergistic Effects of Orbital Shear Stress on In Vitro Growth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells
Table 1
Human primer sequences.
| | Gene | Sequence (5′-3′) | Acc. no. | PCR cycles | Product size |
| GAPDH
| ACCACAGTCCATGCCATCA | NM_002046 | 22 | 452 | | TCCACCACCCTGTTGCTGT |
| COL-1
| CTGACCTTCCTGCGCCTGATGTCC | XM_012651 | 23 | 300 | | GTCTGGGGCACCAACGTCCAAGGG |
| RUNX2
| CGCATTCCTCATCCCAGTAT | NM_001015051 | 30 | 462 | | GACTGGCGGGGTGTAAGTAA |
| OPN
| CCCACAGACCCTTCCAAGTA | J04765 | 29 | 279 | | ACACTATCACCTCGGCCATC |
| OCN
| GTGCAGAGTCCAGCAAAGGT | X53698 | 29 | 175 | | TCAGCCAACTCGTCACAGTC |
| SMAD1
| CAACGCCACTTTTCCAGATT | U59423 | 30 | 313 | | GCACCAGTGTTTTGGTTCCT |
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ALP: alkaline phosphatase; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; COL-I: collagen type I; RUNX2: runt-related transcription factor 2; OPN: osteopontin; OCN: osteocalcin; SMAD1: SMAD family member 1.
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