Rapid and Accurate Detection of Bacteriophage Activity against Escherichia coli O157:H7 by Propidium Monoazide Real-Time PCR

Table 1

Assessment of the extent to which proportion of compromised/damaged E. coli O157:H7 R508N cells interfere with quantification of intact cells as estimated by qPCR.

Live/dead cells (%)	0	30	50	70	100
Live ()	0
Compromised/damaged ()					0
PMA-qPCR
	NA
Concentration	NA
qPCR without PMA
Concentration

NA: not available—did not amplify.
The mean ± standard deviation of R508N concentration was compiled from two independent replicates.