Parkia biglobosa Improves Mitochondrial Functioning and Protects against Neurotoxic Agents in Rat Brain Hippocampal Slices
Figure 2
Effect of PBE (a) and catechin (b) on basal ROS generation in rats’ brain hippocampal slices. Hippocampal slices were incubated in the presence or absence of Parkia biglobosa extract, PBE (25, 50, 100, or 200 g/mL), or catechin (1, 5, or 10 g/mL). Slices of each sample were then homogenized to obtain the supernatant in which ROS production was quantified by reacting with 5 M DCFH-DA. Data of ROS levels are presented as fluorescence intensity emission and expressed as mean ± SEM of three independent assays. Data analysis was done by two-way ANOVA, followed by Bonferroni posttests ( was considered statistically significant). Statistically significant mitigation of basal ROS generation was achieved at 100 and 200 g/mL concentration of PBE (a) and 10 g/mL concentration of catechin (b). and versus untreated slices (control).
