Parkia biglobosa Improves Mitochondrial Functioning and Protects against Neurotoxic Agents in Rat Brain Hippocampal Slices
Figure 7
Effects of PBE and catechin on brain mitochondrial ROS production: SNP and interactions. Mitochondria were incubated in a medium containing 50 M EGTA, 10 mM sucrose, 65 mM KCl, 5 mM glutamate, 5 mM succinate, and 10 mM HEPES, , and PBE (25, 50, or 100 g/mL) or catechin (1, 5, or 10 g/mL) in the presence or absence of 150 M SNP or 80 M for 10 s. The reaction was initiated by the addition of 2′,7′-dichlorofluorescein diacetate (DCFH-DA) and the fluorescence intensity emission arising from the oxidized fluorescent derivative (DCF) was measured over a 300 s period. Results are presented as mean ± SEM of three independent experiments. Data analysis was done by two-way ANOVA, followed by Bonferroni posttests ( was considered statistically significant). Indicators of statistical significance were not shown in the graphs above to avoid ambiguity.
