Antioxidant and Anti-Inflammatory Effects of Selected Natural Compounds Contained in a Dietary Supplement on Two Human Immortalized Keratinocyte Lines
Figure 8
Effect of the seven supplement’s components on HaCaT keratinocyte MCP-1 production. Cells were exposed to increasing concentrations of the components in the absence (a) or in the presence (b) of TNF-α (20 ng/mL, 6 h pretreatment and further 18 h with the components); the two concentrations used for each component are the lowest and highest concentrations reported in Section 2. Data are the means ± SE of a number of determinations ranging from 4 to 6. a: significantly different from control (); b: significantly different from control (), one-way ANOVA followed by Dunnett’s test. (c) Cells were exposed simultaneously to all the components, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiment. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 3 to 6. : significantly different from respective control (, two-tailed unpaired -test). (d) The cells were exposed simultaneously to all the components excluding Se, both in basal conditions or in the presence of TNF-α (20 ng/mL), following the timing of the previous experiments. Each component was used at the highest concentration reported in Section 2. Data are the means ± SE of a number of determinations ranging from 6 to 8. : significantly different from respective control (, two-tailed unpaired t-test).
