Rapid Purification of a New P-I Class Metalloproteinase from Bothrops moojeni Venom with Antiplatelet Activity
Figure 7
Histopathological analysis of myonecrosis induced by B. moojeni or BmooMPα-II. Light micrographs of sections of mouse gastrocnemius muscle 24 hours after i.m. injection of B. moojeni crude venom or BmooMPα-II (50 μg/50 μL saline), stained with hematoxylin-eosin. (a) Control: normal integer fibres are seen. (b-c) and (d-e) B. moojeni crude venom and BmooMPα-II, respectively: note myonecrosis evidenced by loss of cellular boundaries, cytoplasmic changes, no apparent striations and low or absent nuclei, accompanied by inflammatory infiltrate and haemorrhage. Letters indicate the presence of necrosis (N), inflammatory infiltrate (I) and haemorrhage (H). Scale bar = 100 μm (a, b, d); Scale bar = 50 μm (c, e).
