Assessment of Genetic Diversity of Zoonotic Brucella spp. Recovered from Livestock in Egypt Using Multiple Locus VNTR Analysis

Table 1

Brucella spp. isolates recovered from livestock in Egypt (2002–2007) and examined by MLVA-15 technique included in the present study (𝑛=17). Panel 1 includes eight minisatellite markers (Bruce 06–Bruce 55) and Panel 2 is composed of seven microsatellite markers (Bruce 18–Bruce 30). The number of tandem repeats observed in each marker is indicated in the corresponding cells.

Id. strain	Sample	Host	Origin	Year	Specie (biovar)	MLVA-15 Panel 1								MLVA-15 Panel 2
						Bruce 06	Bruce 08	Bruce 11	Bruce 12	Bruce 42	Bruce 43	Bruce 45	Bruce 55	Bruce 18	Bruce 21	Bruce 04	Bruce 07	Bruce 09	Bruce 16	Bruce 30
5	Lymph node	Cow	ND*	2002–2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	9	5	3
6	Fetus	Cow	ND	2002–2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	6	4	3
7	Lymph node	Sheep	Sharkia	2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	7	8	5	7	5	8	3
8	Spleen	Cow	ND	2002–2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	7	8	6	5	8	4	3
9	Lymph node	Cow	ND	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	9	5	3
11	Lymph node	Cow	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	6	4	3
12	Lymph node	Cow	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	9	5	3
15	Lymph node	Cow	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	7	4	3
16	Lymph node	Sheep	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	6	5	9	5	3
17	Lymph node	Goat	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	10	5	3
18	Milk	Buffalo	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	6	4	3
19	Fetus	Cow	Beni-Suef	2006-2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	7	5	9	5	3
20	Milk	Buffalo	Assiut	2007	B. melitensis bv. 3	3	5	3	13	1	1	3	3	8	8	6	5	6	6	3
10	Milk	Cow	Menofia	2007	B. suis bv. 1	2	3	6	10	4	1	5	2	4	9	6	6	5	5	3
14	Lymph node	Cow	Beni-Suef	2006-2007	B. suis bv. 1	2	3	6	10	4	1	5	2	4	9	5	5	8	6	3
13	Spleen	Cow	Beni-Suef	2006-2007	B. abortus	4	5	4	12	2	3	3	3	6	8	3	7	3	3	5
4	Milk	Cow	ND	2002–2007	B. abortus	4	5	4	12	2	3	3	3	6	8	3	9	3	3	5

 *ND: non determined.