Inhibition of Nitric Oxide Synthase Prevents Muscarinic and Purinergic Functional Changes and Development of Cyclophosphamide-Induced Cystitis in the Rat
Figure 6
Representative images of immunohistochemical muscarinic M5 receptor-like (upper row; (a), (b), (c), (d), and (e)), P1A1 purinoceptor-like (middle row; (f), (g), (h), (i), and (j); solid arrows indicate staining) and MIF-like (lower row; (k), (l), (m), (n), and (o); solid arrows indicate staining, dashed arrow indicates possibly occurring staining) staining. Left columns show saline pretreated and saline treated (control), negative ((a), (f), (k)) and positive staining ((b), (g), and (l)). The middle column ((c), (h), and (m)) shows saline pretreated and CYP treated and the second column from the right ((d), (i), and (n)) shows L-NAME pretreated and saline treated bladders. The right column ((e), (j), and (o)) shows staining from L-NAME pretreated and CYP treated bladders. The horizontal bar represents 200 μm for all images. All sections are counterstained with DAPI (blue) and phalloidin (red). Green color represents positive staining.
