Differences in Cytotoxic, Genotoxic, and Inflammatory Response of Bronchial and Alveolar Human Lung Epithelial Cells to Pristine and COOH-Functionalized Multiwalled Carbon Nanotubes
Table 1
Basic properties of the tested MWCNTs.
MWCNTs
MWCNT-COOH
Testing method
Diameter (nm)
Mean 32.0 ± 15
Mean 24.5 ± 10
TEM
Length (m)
Range 0.070–7.80
Range 0.029–1.56
TEM
SSA (m2/g)
106.7
139.1
BET
Z potential in RPMI with 10% FBS (mV)
−9.2 ± 0.5
−10.1 ± 0.4*
Zeta potential analyzer
Z potential in BEGM (mV)
−10.3 ± 0.6
−10.6 ± 0.9
Zeta potential analyzer
Agglomerate/aggregate size in RPMI with 10% FBS (m)
8.1 ± 4.5
4.1 ± 2.1
TEM
Agglomerate/aggregate size in BEGM (m)
5.7 ± 4.6
5.1 ± 2.9
TEM
Agglomerate/aggregate size in RPMI with 10% FBS (diameter Z average nanometers)
1542 ± 291
927 ± 231*
DLS
Agglomerate/aggregate size in BEGM (diameter Z average nanometers)
1841 ± 617
1949 ± 700
DLS
TEM: transmission electron microscopy; BET: Brunauer-Emmett-Teller; SSA: specific surface area; DLS: dynamic light scattering. * MWCNT-COOH versus MWCNTs.
