The Design of a Quantitative Western Blot Experiment

Table 3

Statistical analysis of densitometric data.

FD by time	𝑛	Mean	SE	Pooled SE	SD
𝑇=0	3	0.95	0.189	0.290	0.33
𝑇=1	3	4.17	0.167	0.290	0.29
𝑇=2	3	7.15	0.435	0.290	0.75
Source of variation	Sum squares	DF	Mean square	𝐹 statistic	𝑃
Time	57.70	2	28.85	114.03	<0.0001
Residual	1.52	6	0.25
Total	59.22	8
Tukey contrast	Difference	95% CI
𝑇=0 v 𝑇=1	−3.22	−4.48	to −1.96	(Significant)
𝑇=0 v 𝑇=2	−6.20	−7.46	to −4.94	(Significant)
𝑇=1 v 𝑇=2	−2.98	−4.24	to −1.72	(Significant)