Simple and Cost-Effective Restriction Endonuclease Analysis of Human Adenoviruses

Table 1

Overall time, including preparation time, required for the restriction endonuclease analysis (REA) of HAdVs using 10 samples.

REA steps	Time required
(1) DNA extraction from infected cells	1 hour 30 minutes
(2) Quantification of DNA of genomic DNA and electrophoresis	1 hour 15 minutes
(3) Restriction endonuclease digestion	5–15 min by fast-cut enzyme (1 hour 30 minutes by conventional enzyme)
(4) Agarose gel electrophoresis	1 hour 50 minutes
(5) Gel stain	30 minutes
Total time	Approx. 6 hours