High Molecular Weight Proteins of Trypanosoma cruzi Reduce Cross-Reaction with Leishmania spp. in Serological Diagnosis Tests

Table 2

Glycoproteins present in high molecular weight extracts fraction.

Extraction agent	Lectin	Recognized carbohydrate	Glycoprotein M.W. (kDa) recognized also by chagasic patients’ antibodies
Sonication	Con A	𝛼Man > 𝛼Glc > GlcNAc	>250, 190, 160, 130, 120, 110, and 100
	WGA	GlcNAc, NeuNAc	>250, 190
	Jacalin	𝛼Gal→Ome	>250, 190, 130, 120, 110, and 100
	PT	GalNAc, 𝛽Gal	—
	PNA	𝛽Gal (1→3) galNAc	—
Triton X-100	Con A	𝛼Man > 𝛼Glc > GlcNAc	>250, 170, 160, 140, 120, and 110
	WGA	GlcNAc, NeuNAc	>250
	Jacalin	𝛼Gal→Ome	—
	PT	GalNAc, 𝛽Gal	—
	PNA	𝛽Gal (1→3) galNAc	—
NP40	Con A	𝛼Man > 𝛼Glc > GlcNAc	>250, 170, 150, 120, 115, and 100
	WGA	GlcNAc, NeuNAc	—
	Jacalin	𝛼Gal→Ome	—
	PT	GalNAc, 𝛽Gal	—
	PNA	𝛽Gal (1→3) galNAc	—
Urea-thiourea	Con A	𝛼Man > 𝛼Glc > GlcNAc	>250, 100
	WGA	GlcNAc, NeuNAc	—
	Jacalin	𝛼Gal→Ome	130, 100
	PT	GalNAc, 𝛽Gal	—
	PNA	𝛽Gal (1→3) galNAc	—

Lectin blot assays were performed to determine carbohydrate content in each fraction with a 5 𝜇g/mL concentration of lectins Con A, WGA, Jacalin, PT, PNA, and MAA and the peroxidase-conjugated streptavidin (dilution 1 : 2000).