Research Article

The Structural Modeling of the Interaction between Levofloxacin and the Mycobacterium tuberculosis Gyrase Catalytic Site Sheds Light on the Mechanisms of Fluoroquinolones Resistant Tuberculosis in Colombian Clinical Isolates

Table 1

Primers used to amplify and sequence QRDR-A and QRDR-B regions.

Target regionPrimerNucleotide sequenceMethod

QRDR-AgyrA15F5′-GATGACAGACACGACGTTGC-3′PCR
gyrA19R5′-GCCAGCTCACGCAGGTTG-3′
gyrA17F5′-ATCGACTATGCGATGAGCGTG-3′Sequencing
gyrA18R5′-ATGCCGCCTGACCCGTTG-3′

QRDR-BgyrB5F5′-ACCTTCGCCAACACCATCAACACC-3′PCR and sequencing
gyrB10R5′-CGAACCGAGGGATCCATGGTG-3′
gyrB7F5′-CGGTTCTGCAAAAAGCGGTCGC-3′Sequencing
gyrB8R5′-CGGAAGTATCGCCTGGAACATCG-3′