Preparation of North American Type II PRRSV Infectious Clone Expressing Green Fluorescent Protein

Table 1

Primers for amplifying gfp gene fused with PRRSV Nsp2 gene sequence.

Primers	Sequence of primers (5^′→3^′)	Sizes/bp	Location in the plasmids
F1	CTACTATCCT GCACAAGGTG	355	2351~2370 (FL12)
R1	CAGGCCGCTCTCGTCGCTCTCTGGCAAGCTCTTGACAGACTTTG		9854~9875 (pCDNA-EF1-GFP); 2662~2684 (FL12)
F2	CAAAGTCTGTCAAGAGCTTGCCAGAGAGCGACGAGAGCGGCCTG	800	2662~2684 (FL12); 9854~9875 (pCDNA-EF1-GFP)
R2	GCGGGGACAGGTTTGTTCCCGCGAGATCCGGTGGAGCC		2684~2704 (FL12); 10589~10607 (pCDNA-EF1-GFP)
F3	GGCTCCACCGGATCTCGCGGGAACAAACCTGTCCCCGC	840	10589~10607 (pCDNA-EF1-GFP); 2684~2704 (FL12)
R3	GGTGTCTCGAGTATCATCTTTG		3481~3502 (FL12)