miR-375 Suppresses IGF1R Expression and Contributes to Inhibition of Cell Progression in Laryngeal Squamous Cell Carcinoma
Figure 6
miR-375 inhibited the expression of IGF1R by targeting its 3′-UTR. (a) Schematic diagram showing the two potential targeting sites of miR-375 in the 3′-UTR of IGF1R and gene sequence included in four luciferase reporter plasmids, respectively. (b–e) Relative luciferase activities of IGF1R-wt and IGF1R-mut reporters were obtained by cotransfection of scrambled control miRNA, miR-375 mimic or miR-375 inhibitor, and psiCHECK-2-IGF1R-3′UTR reporter plasmid and calculated as the ratio of Firefly/Renilla activities and normalized to those of the control. () Significant difference when compared with the control (). () Significant difference when compared with the control ().
