Complete Cell Killing by Applying High Hydrostatic Pressure for Acellular Vascular Graft Preparation

Figure 3

Morphology of cells after pressure treatment at 24 hours. (a–e) NIH/3T3, (f–j) smooth muscle, and (k–o) endothelial cells are shown as phase-contrast images. The cells were treated with the pressure of 0 (a, f, and k), 100 (b, g, and l), 200 (c, h, and m), 500 (d, i, and n), and 1000 (e, j, and o) for 10 min. The cells treated at 100 MPa were completely adhered on the culture dish as that of untreated cells.

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