|
| Primer name | Primer sequence (5′-3′) | Annealing temperature | Amplicon size (bp) |
|
| Semiquantitative RT-PCR | | | |
| hsp70 | F: 5′-GCATGGTGAACCACTTTGTG-3′ | 53°C | 368 |
| R: 5′-CTCTGCCGTTGAAGAAATCC-3′ |
| hsp78 | F: 5′-TCCAGGCTACCCTGATTTTG-3′ | 60°C | 360 |
| R: 5′-CACCATGCCTACATCCTCCT-3′ |
| hsp60 | F: 5′-C(C/T)G TCA CCATGG G(A/G/T)C CAA AGG-3′ | 65°C | 648 |
| R: 5′-(C/G/T)GC CTC TCC ATC CAC ATC C/T)TC(A/C)GC-3′ |
| hsp90 | F: 5′-GGAAATCTTCCTCCGAGAGC-3′ | 51°C | 244 |
| R: 5′-CCGAATTGACCGATCATAGA-3′ |
| hsp110 | F: 5′-GCAGATCTCCGCTATGCTGT-3′ | 56°C | 242 |
| R: 5′-CGAATGACCCAAGTCCACGA-3′ |
| hsp47 | F: 5′-CACTGGGATGAGAAGTTCCA-3′ | 59°C | 408 |
| R: 5′-AAGGAAAATGAAGGGATGGTC-3′ |
| hsp27 | F: 5′-CTT TGG GAT GCC ACA CTT CT-3′ | 50°C | 233 |
| R: 5′-ATC TGG CGT CTG CTT GAT CT-3′ |
|
| Quantitative RT-PCR | | | |
| hsp70 | F: 5′-GTC GTG GATCTGTCCCTTGT-3′ | 57°C | 98 |
| R: 5′-CTCGCTTTGAGGAGCTGTG-3′ |
| hsp78 | F: 5′-GTC TTG GTG GGT GGC TCT AC-3′ | 57°C | 94 |
| R: 5′-TCA GGG TTG ATG CCC CTA GA-3′ |
| hsp60 | F: 5′-TCG CAC TGT CAT CAT TGA GC-3′ | 61°C | 91 |
| R: 5′-CTT GTC TTT CAG GTC GAT GG-3′ |
| hsp90 | F: 5′-CTG GGG TCT GTC AAG CTT TC-3′ | 60°C | 122 |
| R: 5′-AGG CCT TGG ACA AAA TCA GA-3′ |
| hsp110 | F: 5′-AAG CTG GAG GGC TCC TAT CA-3′ | 53°C | 90 |
| R: 5′-ATA CAG CCA GTT CTC CGT GC-3′ |
| hsp47 | F: 5′-CTG ACA GAG GCT GTG GAC AA-3′ | 52°C | 60 |
| R: 5′-GTC CTT CTT CCC GGA GAT GT-3′ |
| hsp27 | F: 5′-TGG GAT GCC ACA CTT CTC CG-3′ | 59°C | 70 |
| R: 5′-GAT GTA TCC AGG CCA GTG CG-3′ |
|
