Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells
Figure 6
Time dependent effect of miR-146a on apoptosis in HCC cell lines. HepG2, HepB3, and SNU449 cells (2.5 × 103 cells per well in 96-well plate) were cultured for 24 h and then transfected with miR-146a inhibitor, miR-146a mimic, EGFR siRNA, and their negative controls (200 nM) up to another 96 h. Hoechst 33342/propidium iodide (PI) double fluorescent chromatin staining was used to analyze apoptosis. , , compared to blank and negative controls at the same time point.