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BioMed Research International
Volume 2014, Article ID 390506, 9 pages
http://dx.doi.org/10.1155/2014/390506
Research Article

Establishment of a Swine Model for Validation of Perfusion Measurement by Dynamic Contrast-Enhanced Magnetic Resonance Imaging

1Radiation and Tumor Clinic, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany
2Evangelisches Krankenhaus Wesel GmbH, Schermbecker Landstraße 88, 46485 Wesel, Germany
3Central Animal Laboratory, Essen University Hospital, Hufelandstraße. 55, 45147 Essen, Germany
4Department of Small Animal Surgery, Hospital of Veterinary Medicine, Justus Liebig University Giessen, Frankfurter Straße 94, 35392 Gießen, Germany
5Charité Universiätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany

Received 18 December 2013; Accepted 20 January 2014; Published 27 February 2014

Academic Editor: Fabio Minutoli

Copyright © 2014 Anika Sauerbrey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was to develop a suitable animal model for validating dynamic contrast-enhanced magnetic resonance imaging perfusion measurements. A total of 8 pigs were investigated by DCE-MRI. Perfusion was determined on the hind leg musculature. An ultrasound flow probe placed around the femoral artery provided flow measurements independent of MRI and served as the standard of reference. Images were acquired on a 1.5 T MRI scanner using a 3D T1-weighted gradient-echo sequence. An arterial catheter for local injection was implanted in the femoral artery. Continuous injection of adenosine for vasodilation resulted in steady blood flow levels up to four times the baseline level. In this way, three different stable perfusion levels were induced and measured. A central venous catheter was used for injection of two different types of contrast media. A low-molecular weight contrast medium and a blood pool contrast medium were used. A total of 6 perfusion measurements were performed with a time interval of about 20–25 min without significant differences in the arterial input functions. In conclusion the accuracy of DCE-MRI-based perfusion measurement can be validated by comparison of the integrated perfusion signal of the hind leg musculature with the blood flow values measured with the ultrasound flow probe around the femoral artery.