Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
Figure 2
Secretion of rBLG by NZ9000. Two rNZ9000 strains were cultured with (+) or without (−) 10 ng/mL of nisin. Protein extracts from cells (a) or culture supernatants (b) were analyzed by Western blotting with a 6x His-tag Ab. White and black arrowheads indicate the secretory rBLG precursor (pre-rBLG, 27 kDa) and the secretory form of rBLG (24.3 kDa), respectively.