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BioMed Research International
Volume 2014 (2014), Article ID 395781, 10 pages
http://dx.doi.org/10.1155/2014/395781
Research Article

Direct Cell-Cell Contact between Mesenchymal Stem Cells and Endothelial Progenitor Cells Induces a Pericyte-Like Phenotype In Vitro

1AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos, Switzerland
2Department of Trauma Surgery, Regensburg University Medical Center, 93042 Regensburg, Germany
3Department of Oral and Maxillofacial Surgery, Albert-Ludwigs-University, 79106 Freiburg, Germany

Received 4 October 2013; Revised 24 November 2013; Accepted 15 December 2013; Published 20 January 2014

Academic Editor: Sanga Gehmert

Copyright © 2014 Markus Loibl et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Gene regulation in Depleted-MSCs and EPCs after 3 days of culture in IMDM-FCS. Pericyte marker genes CD146 (A, F), NG2 (B, G), αSMA (C, H), and PDGFR-β (D, I) and endothelial marker gene PECAM-1 (E, J) were detected in depleted-MSCs and EPCs after 3 days in transwell culture, direct coculture and single culture in IMDM-FCS (IMDM supplemented with 10% FCS). Results are presented for each gene as relative change in gene expression (2-ΔΔCt) over time between day 0 and day 3 for transwell culture, direct co-culture, and single culture in 5 independent experiments (donor 1-5), and as mean ± error of the mean of all experiments.

Gene regulation in Depleted-MSCs and EPCs after 3 days of culture in IMDM-PL. Pericyte marker genes CD146 (A, F), NG2 (B, G), αSMA (C, H), and PDGFR-β (D, I) and endothelial marker gene PECAM-1 (E, J) were detected in depleted-MSCs and EPCs after 3 days in transwell culture, direct coculture and single culture in IMDM-PL (IMDM supplemented with 5% FCS, 5% PL). Results are presented for each gene as relative change in gene expression (2-ΔΔCt) over time between day 0 and day 3 for transwell culture, direct coculture, and single culture in 5 independent experiments (donor 1-5) and as mean ± standard error of the mean of all experiments.

Gene regulation in MSCs, Depleted-MSCs and corresponding EPCs after 3 days of culture in IMDM-FCS and IMDM-PL. Pericyte marker genes CD146, NG2, αSMA, and PDGFR-β, and endothelial marker gene PECAM-1 were detected in MSCs, depleted-MSCs and corresponding EPCs after 3 days in transwell culture, direct coculture and single culture in IMDM-FCS (IMDM supplemented with 10% FCS) and IMDM-PL (IMDM supplemented with 5% FCS, 5% PL). Results are presented for each gene as relative change in gene expression (2-ΔΔCt) over time between day 0 and day 3 for transwell culture, coculture, and single culture as mean ± standard error of the mean of 5 independent experiments.

  1. Supplementary Figure 1
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  3. Supplementary Table 1