Research Article

Sequence and Apoptotic Activity of VacA Cytotoxin Cloned from a Helicobacter pylori Thai Clinical Isolate

Figure 3

DAPI staining of intestinal epithelial cells (T84) and Madin-Darby Canine Kidney (MDCK) cells. (a) Control of untreated T84 cells showing normal nuclei. (b) T84 cells treated with VacA (150 µg mL−1) cells showing chromatin condensation and DNA fragmentation. (c) Control of untreated MDCK cells. (d) MDCK cells treated with VacA (150 µg mL−1) showing chromatin condensation and DNA fragmentation. DAPI was used at 1000-fold dilution in buffer and the magnification of images is 1000×.
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