RhoA Controls Wnt Upregulation on Microstructured Titanium Surfaces

Figure 2

(a)-(b) Immunocytochemistry of Myosin II distribution in MC3T3 cells on Polished or SLA surfaces (20x magnification, bar 20 mm, insets: 40x magnification). Heat maps were generated using ImageJ freeware software. (c) Total RhoA activation levels in MC3T3 cells growing on Polished or SLA titanium surfaces by G-LISA assay after Myosin II inhibition by 1 mM Blebbistatin. ^∗𝑃<0.01 versus vehicle-treated SLA, 𝑃<0.001 versus Blebbistatin-treated Polished. (d) Canonical WNT activity by Luciferase reporter assay in C2C12 cells on Polished or SLA surfaces after Myosin II inhibition by 1 mM Blebbistatin. ^∗𝑃<0.01 versus vehicle-treated Polished and Blebbistatin-treated SLA.

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