Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 401859, 9 pages
http://dx.doi.org/10.1155/2014/401859
Research Article

RhoA Controls Wnt Upregulation on Microstructured Titanium Surfaces

1Section of Periodontology and Implant Dentistry, Centro di Odontoiatria, University of Parma, Via Gramsci 14, 43126 Parma, Italy
2Department of Clinical and Experimental Medicine, University of Parma, Via Gramsci 14, 43126 Parma, Italy
3Department of Biomedical, Biotechnological, and Translational Sciences, University of Parma, Via Gramsci 14, 43126 Parma, Italy

Received 28 January 2014; Accepted 20 March 2014; Published 14 May 2014

Academic Editor: H. C. Van der Mei

Copyright © 2014 Simone Lumetti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. C. Galli, G. Guizzardi, G. Passeri et al., “Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces,” Journal of Periodontology, vol. 76, no. 3, pp. 364–372, 2005. View at Publisher · View at Google Scholar · View at Scopus
  2. B. G. Keselowsky, L. Wang, Z. Schwartz, A. J. Garcia, and B. D. Boyan, “Integrin α5 controls osteoblastic proliferation and differentiation responses to titanium substrates presenting different roughness characteristics in a roughness independent manner,” Journal of Biomedical Materials Research A, vol. 80, no. 3, pp. 700–710, 2007. View at Publisher · View at Google Scholar · View at Scopus
  3. I. Wall, N. Donos, K. Carlqvist, F. Jones, and P. Brett, “Modified titanium surfaces promote accelerated osteogenic differentiation of mesenchymal stromal cells in vitro,” Bone, vol. 45, no. 1, pp. 17–26, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. K. A. Kilian, B. Bugarija, B. T. Lahn, and M. Mrksich, “Geometric cues for directing the differentiation of mesenchymal stem cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 11, pp. 4872–4877, 2010. View at Publisher · View at Google Scholar · View at Scopus
  5. C. Galli, G. Passeri, F. Ravanetti, E. Elezi, M. Pedrazzoni, and G. M. MacAluso, “Rough surface topography enhances the activation of Wnt/β-catenin signaling in mesenchymal cells,” Journal of Biomedical Materials Research A, vol. 95, no. 3, pp. 682–690, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. R. Olivares-Navarrete, S. L. Hyzy, D. L. Hutton et al., “Direct and indirect effects of microstructured titanium substrates on the induction of mesenchymal stem cell differentiation towards the osteoblast lineage,” Biomaterials, vol. 31, no. 10, pp. 2728–2735, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. N. Chakravorty, S. Hamlet, A. Jaiprakash et al., “Pro-osteogenic topographical cues promote early activation of osteoprogenitor differentiation via enhanced TGFbeta, Wnt, and Notch signaling,” Clinical Oral Implants Research, 2013. View at Google Scholar
  8. J. Y. Martin, D. D. Dean, D. L. Cochran, J. Simpson, B. D. Boyan, and Z. Schwartz, “Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces,” Clinical Oral Implants Research, vol. 7, no. 1, pp. 27–37, 1996. View at Google Scholar · View at Scopus
  9. S. M. Bang, H. J. Moon, Y. D. Kwon, J. Y. Yoo, A. Pae, and I. K. Kwon, “Osteoblastic and osteoclastic differentiation on SLA and hydrophilic modified SLA titanium surfaces,” Clinical Oral Implants Research, 2013. View at Google Scholar
  10. S. Zinelis, N. Silikas, A. Thomas, K. Syres, and G. Eliades, “Surface characterization of SLActive dental implants,” European Journal of Esthetic Dentistry, vol. 7, no. 1, pp. 72–92, 2012. View at Google Scholar
  11. O. Pertz, L. Hodgson, R. L. Klemke, and K. M. Hahn, “Spatiotemporal dynamics of RhoA activity in migrating cells,” Nature, vol. 440, no. 7087, pp. 1069–1072, 2006. View at Publisher · View at Google Scholar · View at Scopus
  12. D. Stamenović and M. F. Coughlin, “The role of prestress and architecture of the cytoskeleton and deformability of cytoskeletal filaments in mechanics of adherent cells: a quantitative analysis,” Journal of Theoretical Biology, vol. 201, no. 1, pp. 63–74, 1999. View at Publisher · View at Google Scholar · View at Scopus
  13. R. Olivares-Navarrete, S. L. Hyzy, J. H. Park et al., “Mediation of osteogenic differentiation of human mesenchymal stem cells on titanium surfaces by a Wnt-integrin feedback loop,” Biomaterials, vol. 32, no. 27, pp. 6399–6411, 2011. View at Publisher · View at Google Scholar · View at Scopus
  14. D. A. Glass II, P. Bialek, J. D. Ahn et al., “Canonical Wnt signaling in differentiated osteoblasts controls osteoclast differentiation,” Developmental Cell, vol. 8, no. 5, pp. 751–764, 2005. View at Publisher · View at Google Scholar · View at Scopus
  15. S. J. Rodda and A. P. McMahon, “Distinct roles for Hedgehog and caronical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors,” Development, vol. 133, no. 16, pp. 3231–3244, 2006. View at Publisher · View at Google Scholar · View at Scopus
  16. N. Wang and D. E. Ingber, “Control of cytoskeletal mechanics by extracellular matrix, cell shape, and mechanical tension,” Biophysical Journal, vol. 66, no. 6, pp. 2181–2189, 1994. View at Google Scholar · View at Scopus
  17. C. Galli, S. Guizzardi, G. Passeri, G. M. Macaluso, and R. Scandroglio, “Life on the wire: on tensegrity and force balance in cells,” Acta Biomedica de l'Ateneo Parmense, vol. 76, no. 1, pp. 5–62, 2005. View at Google Scholar · View at Scopus
  18. D. E. Ingber, “Tensegrity I. Cell structure and hierarchical systems biology,” Journal of Cell Science, vol. 116, no. 7, pp. 1157–1173, 2003. View at Publisher · View at Google Scholar · View at Scopus
  19. C. S. Chen, M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber, “Geometric control of cell life and death,” Science, vol. 276, no. 5317, pp. 1425–1428, 1997. View at Publisher · View at Google Scholar · View at Scopus
  20. D. Stamenovi, S. M. Mijailovich, I. M. Toli-Nørrelykke, J. Chen, and N. Wang, “Cell prestress—II. Contribution of microtubules,” American Journal of Physiology. Cell Physiology, vol. 282, no. 3, pp. C617–C624, 2002. View at Google Scholar · View at Scopus
  21. M. Endo, M. Nishita, and Y. Minami, “Analysis of wnt/planar cell polarity pathway in cultured cells,” Methods in Molecular Biology, vol. 839, pp. 201–214, 2012. View at Publisher · View at Google Scholar · View at Scopus
  22. C. Gao and Y.-G. Chen, “Dishevelled: the hub of Wnt signaling,” Cellular Signalling, vol. 22, no. 5, pp. 717–727, 2010. View at Google Scholar · View at Scopus
  23. A. J. Mikels and R. Nusse, “Purified Wnt5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context,” PLoS Biology, vol. 4, no. 4, Article ID e115, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. Y.-N. Lee, Y. Gao, and H.-Y. Wang, “Differential mediation of the Wnt canonical pathway by mammalian Dishevelleds-1, -2, and -3,” Cellular Signalling, vol. 20, no. 2, pp. 443–452, 2008. View at Publisher · View at Google Scholar · View at Scopus
  25. J. D. Axelrod, J. R. Miller, J. M. Shulman, R. T. Moon, and N. Perrimon, “Differential recruitment of dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways,” Genes and Development, vol. 12, no. 16, pp. 2610–2622, 1998. View at Google Scholar · View at Scopus
  26. K. A. Wharton Jr., “Runnin' with the Dvl: proteins that associate with Dsh/Dvl and their significance to Wnt signal transduction,” Developmental Biology, vol. 253, no. 1, pp. 1–17, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. W. J. Pan, S. Z. Pang, T. Huang, H. Y. Guo, D. Wu, and L. Li, “Characterization of function of three domains in dishevelled 1: DEP domain is responsible for membrane translocation of dishevelled-1,” Cell Research, vol. 14, no. 4, pp. 324–330, 2004. View at Publisher · View at Google Scholar · View at Scopus
  28. A. Calzado-Martín, A. Méndez-Vilas, M. Multigner et al., “On the role of RhoA/ROCK signaling in contact guidance of bone-forming cells on anisotropic Ti6Al4V surfaces,” Acta Biomaterialia, vol. 7, no. 4, pp. 1890–1901, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. Y. Sun, L. G. Villa-Diaz, R. H. Lam, W. Chen, P. H. Krebsbach, and J. Fu, “Mechanics regulates fate decisions of human embryonic stem cells,” PLoS ONE, vol. 7, no. 5, Article ID e37178, 2012. View at Google Scholar
  30. H. J. Kwon, “Chondrogenesis on sulfonate-coated hydrogels is regulated by their mechanical properties,” Journal of the Mechanical Behavior of Biomedical Material, vol. 17, pp. 337–346, 2013. View at Google Scholar
  31. D. W. Dumbauld, H. Shin, N. D. Gallant, K. E. Michael, H. Radhakrishna, and A. J. García, “Contractility modulates cell adhesion strengthening through focal adhesion kinase and assembly of vinculin-containing focal adhesions,” Journal of Cellular Physiology, vol. 223, no. 3, pp. 746–756, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. Y. Fonar, Y. E. Gutkovich, H. Root et al., “Focal adhesion kinase protein regulates Wnt3a gene expression to control cell fate specification in the developing neural plate,” Molecular Biology of the Cell, vol. 22, no. 13, pp. 2409–2421, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. M. Despeaux, G. Chicanne, E. Rouer et al., “Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered Wnt signaling,” Stem Cells, vol. 30, no. 8, pp. 1597–1610, 2012. View at Google Scholar
  34. C. H. Seo, K. Furukawa, K. Montagne, H. Jeong, and T. Ushida, “The effect of substrate microtopography on focal adhesion maturation and actin organization via the RhoA/ROCK pathway,” Biomaterials, vol. 32, no. 36, pp. 9568–9575, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. B. K. Teo, S. T. Wong, C. K. Lim et al., “Nanotopography modulates mechanotransduction of stem cells and induces differentiation through focal adhesion kinase,” ACS Nano, 2013. View at Google Scholar
  36. C. Higuchi, N. Nakamura, H. Yoshikawa, and K. Itoh, “Transient dynamic actin cytoskeletal change stimulates the osteoblastic differentiation,” Journal of Bone and Mineral Metabolism, vol. 27, no. 2, pp. 158–167, 2009. View at Publisher · View at Google Scholar · View at Scopus