Supplementation with Sodium Selenite and Selenium-Enriched Microalgae Biomass Show Varying Effects on Blood Enzymes Activities, Antioxidant Response, and Accumulation in Common Barbel (Barbus barbus)

Table 5

Oxidative stress and antioxidant responses in tissue of common barbel (Barbus barbus L.) yearlings fed on control diet containing Se-free microalgae biomass and on diets supplemented by either sodium selenite or Se-enriched microalgae (replacing Se-free microalgae) to obtain Se concentrations of 0.3 mg kg⁻¹ from sodium selenite (Se 0.3) or 0.3 and 1.0 mg kg⁻¹ from Se-enriched microalgae biomass Algadiet 0.3 and Algadiet 1.0, respectively.

Indices	Tissues	Test groups
		Control	Se 0.3	Algadiet 0.3	Algadiet 1.0
TBARS (nmol g ww^−1)	Muscle	8.4±3.8^a	7.5±4.4^a	7.1±3.6^a	6.5±3.6^a
	Liver	32.8±9.1^a	33.3±8.3^a	32.3±9.8^a	31.4±8.1^a
	Intestine	3.4±1.1^a	3.1±0.9^a	2.9±0.8^a	2.8±0.9^a
SOD (U mg^−1 protein)	Muscle	21.9±4.5^a	19.3±5.4^a	20.5±5.4^a	21.4±4.2^a
	Liver	141.9±21.8^a	127.1±21.2^a	137.0±15.3^a	164.0±26.3^a
	Intestine	5.1±1.6^a	4.8±1.1^a	5.0±1.3^a	4.3±1.2^a
CAT (mU mg^−1 protein)	Muscle	2.4±0.8^a	3.7±0.8^b	2.7±1.0^a	4.6±1.1^c
	Liver	26.9±8.1^a	41.7±8.6^bc	35.4±7.7^b	47.1±9.6^c
	Intestine	0.8±0.3^a	0.9±0.3^a	0.8±0.2^a	0.8±0.2^a
GPx (U mg^−1 protein)	Muscle	66.4±14.9^a	63.0±10.7^a	64.8±9.4^a	62.5±8.5^a
	Liver	194.4±45.1^a	185.6±35.4^a	177.4±32.9^a	198.2±26.0^a
	Intestine	29.6±6.6^a	29.5±7.6^a	27.9±6.4^a	25.0±6.3^a
GR (U mg^−1 protein)	Muscle	17.4±7.3^b	7.3±3.2^a	10.0±3.6^a	6.8±2.8^a
	Liver	6.0±2.3^a	6.9±2.0^a	6.2±2.3^a	6.0±2.6^a
	Intestine	8.1±2.0^a	7.7±2.7^a	7.1±2.6^a	6.7±2.5^a

Different superscripts in the same row indicate significant differences 𝛼=0.01; 𝑛=8 per replication.