Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells
Figure 3
hUCB-CD34+ cells cocultured with AFT024-hkirre and CFU assay. (a) AFT024-hkirre feeder cells at 100% confluence. (b) Picture of AFT024-hkirr cells cocultured with CD34+ cells. Picture was made on the 7th day of coculture. The bright contrast cells are attached or floating, and dark contract cells are underneath migrated HSCs. (c) The picture of CFU-Mix, showing the granulocytic and erythrocytic colonies. (d) The picture of CFU-GM on day 16. All pictures were taken at 100x.
