BioMed Research International / 2014 / Article / Fig 3

Research Article

TBT Effects on the Development of Intersex (Ovotestis) in Female Fresh Water Prawn Macrobrachium rosenbergii

Figure 3

(a) Cross-section through ovary of prawn (control) showing vitellogenic oocytes with distinct ooplasm (OP) filled with yolk globules (Yg) with nucleus (N). Each oocyte is enveloped by a row of characteristic follicle cells (FC). (b) At 10 ng/L TBT exposure, ovary showing previtellogenic oocytes (IO) and follicle cells (FC). (c) Ovary showing the fusion of developing oocytes ( DO) and increase in interstitial connective tissues (ICT) at 100 ng/L TBT exposure and (d) At 1000 ng/L TBT exposure, ovary showing immature oocytes and increase in interstitial connective tissues (ICT). (e) Higher magnification of the above, showing ovotestis formation with spermatogonia (Sg), increase in interstitial connective tissues (ICT), and immature oocytes (IO). (f) And (g) ovary showing connective tissues modified into tubular like structure ( ) and increase in interstitial connective tissues ( ICT). (h) Ovary of prawn treated with TBT (1000 mg/L) showing occurrence of spermatogonia (Sg) in the ovary. Bar: 50 μm.

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