The Influence of Simulated Microgravity on Purinergic Signaling Is Different between Individual Culture and Endothelial and Smooth Muscle Cell Coculture

Figure 7

Scheme of P2 Receptor Alteration and the Postulated Paracrine Effect in ECs and SMCs under Simulated Microgravity. Several P2 receptor expressions were altered in ECs and SMCs under 24 h simulated microgravity condition using a clinostat. To point out that the expression P2X7 and P2Y2 was altered differentially between ECs and SMCs under simulated microgravity. Especially, the change of P2X7 in ECs was compensated under SMC-conditioned medium and vice versa. The conditioned medium collected under simulated microgravity showed the pathogenic influence of EC and SMC proliferation and migration if compared to condition medium from normal gravity.