Caspase-14 Expression Impairs Retinal Pigment Epithelium Barrier Function: Potential Role in Diabetic Macular Edema
FITC-dextran flux assay. Permeability is defined by (cm/s). There was a significant increase in permeability of cells cultured under high glucose (HG) conditions or transfected with caspase-14 plasmid at different time points (1, 3, and 6 h) compared with cells grown under normal glucose (NG) conditions or HG-treated cells transfected with caspase-14 siRNA (). Caspase-14 siRNA is significantly lower than high glucose at 3 h and 6 h (). Please note that caspase-14 plasmid has the same permeability effect as HG at 3 and 6 h (, versus control and HG + caspase-14 siRNA, versus control plasmid).