Research Article

Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR

Table 2

Comparison of detection of respiratory virus by three automatic extraction methods from nasopharyngeal swab specimens.

VirusQIAcubeEZ1 advanced XLMICROLAB Nimbus IVDTotal number of specimens

RV+++14
++3
+1a
++1
+2b

MPV +++7
++1

PIV3+++8

PIV1+++1
+1b

PIV4+1b

RSV-A+++7
+1b

ADV+++2
++1
++2
+3b

FluB+++2
+1a

FluA+++2

BoV+++1
+1a

EV+++2
+1b

CoV-OC43 11

CoV-NL63+1b

CoV-229E+1b

Total57595468

Virus detected by only 1 of the three automated nucleic acid extraction systems, and we confirmed this result by sequencing analysis and confirmed it as “true positive.”
bVirus detected by only 1 of the three automated nucleic acid extraction systems, but they were not detected by repeated test with the Seeplex RV15 ACE Detection kit (Seegene).