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BioMed Research International
Volume 2014 (2014), Article ID 436206, 6 pages
http://dx.doi.org/10.1155/2014/436206
Research Article

The Kampo Medicine Rokumigan Possesses Antibiofilm, Anti-Inflammatory, and Wound Healing Properties

1Oral Ecology Research Group, Faculty of Dentistry, Laval University, 2420 Rue de la Terrasse, Quebec City, QC, Canada G1V 0A6
2Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China
3Department of Oral Health Science and Social Welfare, Institute of Health Biosciences, The University of Tokushima Graduate School, 18-15 Kuramotocho 3-Chome, Tokushima 770-8504, Japan
4Department of Hygiene and Oral Health Science, Institute of Health Biosciences, The University of Tokushima Graduate School, 18-15 Kuramotocho 3-Chome, Tokushima 770-8504, Japan

Received 27 February 2014; Accepted 9 April 2014; Published 30 April 2014

Academic Editor: Toshihide Kurihara

Copyright © 2014 James Liao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Periodontal diseases, which are inflammatory diseases of bacterial origin affecting the tooth-supporting tissues, are characterized by inflammation and destruction of gingival connective tissue and alveolar bone, and may lead to tooth loss. The aim of the study was to investigate Rokumigan, a Kampo Japanese traditional medicine made of six different plants, for its capacity to prevent biofilm formation by Fusobacterium nucleatum, to inhibit interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion by mucosal cells, and to promote wound healing in a fibroblast model. Using a microplate colorimetric assay, Rokumigan prevented biofilm formation by F. nucleatum, while it had no effect on bacterial growth. Rokumigan inhibited IL-6 secretion in both epithelial cells and fibroblasts stimulated with lipopolysaccharide. However, it caused no significant inhibition of IL-8 secretion by both cell types. Rokumigan significantly increased proliferation and migration of gingival fibroblasts in a wound healing assay. In conclusion, the Kampo formulation Rokumigan, through suppression of biofilm formation by F. nucleatum, inhibition of IL-6 secretion by gingival epithelial cells and fibroblasts, and promotion of wound healing in a fibroblast model, may have potential application for periodontal diseases.