Development of Pathological Diagnostics of Human Kidney Cancer by Multiple Staining Using New Fluorescent Fluolid Dyes
Figure 1
Immunostaining of renal tumors. Antibodies against Kank1 (αKank1), CK7 (αCK7), and CD10 (αCD10) were used as the primary antibodies, and Fluolid-Green-conjugated donkey anti-mouse IgG, Fluolid-Orange-conjugated donkey anti-rabbit IgG, and Alexa Fluor 647-conjugated donkey anti-goat IgG were used, respectively, as the secondary antibodies. DAPI was used to stain nuclei and tissues were histopathologically examined by hematoxylin and eosin (HE) staining. Results of immunostaining of normal tissue (a), clear cell RCC (b), papillary RCC (c), chromophobe RCC (d), renal angiomyolipoma (e), and acquired cystic disease-associated RCC (f) are shown. Note that the parts shown by HE staining in cancer do not match with those shown by immunostaining. Image magnification: 40 for immunostaining in (a)–(f) and HE staining in (a); 20 for HE staining in (b)–(f). Bar = 20 μm.
