437871.fig.002a
(a)
437871.fig.002b
(b)
Figure 2: Stability of fluorescent dyes in histopathological sections. Fluorescent images were examined for a tissue section before (a) or after (b) the storage at room temperature for almost three years. A normal human kidney tissue section was subjected to quadruple staining with three different Fluolid dyes and DAPI. Antibodies against Kank1, CK7, and CD10 were used as the primary antibodies, and Fluolid-Green-conjugated anti-mouse IgG, Fluolid-Orange-conjugated anti-rabbit IgG, and Alexa Fluor 647-conjugated anti-goat IgG were used as the secondary antibodies. Bar = 20 μm.